Formative assessment consists of a qualitative verification of the skills and competencies of an individual in a formal instructional process. This evaluation is fundamental for the progress of the tutorial groups, present in the methodology of Problem-Based Learning, widely used in medical courses in Brazil. However, factors such as lack of knowledge and fear of the evaluation process prevent it from being carried out. Therefore, this study aims to analyze how formative assessment occurs in tutorial groups, based on Emotional Didactic Suitability. That is, it aims to understand how formative assessment affects the interest, attitude and emotions of students participating in the tutorial group. La investigación se caracteriza por ser cualitativa, teniendo como informantes a estudiantes y a un tutor del curso de Medicina de una universidad del interior de Bahía.Para la recolección de datos, se utilizó la Observación Participante utilizando cuestionarios estructurados y observación de dinámicas tutoriales en 2021. Data analysis was performed through the Criterion of Emotional Didactic Suitability adapted to the medical course, which aims to measure how students are interested and affected in the teaching-learning process. As a result, it was noticed that students and teachers still value summative evaluation more than formative evaluation, creating aversive behaviors to the evaluation process, such as anxiety and fear. The notion of the importance of evaluation at the end of tutoring was perceived as a way to stimulate the development of skills and attitudes, although it does not follow a standardization of self-evaluation, peer evaluation, tutor evaluation and tutor evaluation.

La evaluación formativa consiste en una verificación cualitativa de las habilidades y competencias de un individuo en un proceso formal de instrucción. Esta evaluación es fundamental para el progreso de los grupos tutoriales, presentes en la metodología del Aprendizaje Basado en Problemas, ampliamente utilizada en los cursos de medicina en Brasil. Sin embargo, factores como el desconocimiento y el miedo al proceso de evaluación impiden que se lleve a cabo. Por lo tanto, este estudio tiene como objetivo analizar cómo se produce la evaluación formativa en grupos tutoriales, basada en la Idoneidad Didáctica Emocional. Es decir, pretende comprender cómo afecta la evaluación formativa al interés, la actitud y las emociones de los estudiantes que participan en el grupo tutorial. La investigación se caracteriza por ser cualitativa, teniendo como informantes a estudiantes y a un tutor del curso de Medicina de una universidad del interior de Bahía.Para la recolección de datos, se utilizó la Observación Participante utilizando cuestionarios estructurados y observación de dinámicas tutoriales en 2021. El análisis de los datos se realizó a través del Criterio de Idoneidad Didáctica Emocional adaptado al curso de medicina, que tiene como objetivo medir cómo los estudiantes están interesados y afectados en el proceso de enseñanza-aprendizaje. Como resultado, se notó que los estudiantes y los maestros todavía valoran la evaluación sumativa más que la evaluación formativa, creando comportamientos aversivos al proceso de evaluación, como la ansiedad y el miedo. La noción de la importancia de la evaluación al final de la tutoría fue percibida como una forma de estimular el desarrollo de habilidades y actitudes, aunque no sigue una estandarización de autoevaluación, evaluación por pares, evaluación del tutor y evaluación del tutor.

A avaliação formativa consiste em uma verificação qualitativa das habilidades e competências de um indivíduo em processo instrucional formal. Essa avaliação constitui-se como fundamental para o andamento dos grupos tutoriais, presentes na metodologia da Aprendizagem Baseada em Problemas, utilizada amplamente nos cursos de Medicina do Brasil. Porém, fatores como a falta de conhecimento e medo do processo avaliativo impedem que ela seja efetivada. Portanto, esse estudo visa analisar como ocorre a avaliação formativa em grupos tutoriais, com base na Idoneidade Didática Emocional. Ou seja, pretende compreender como a avaliação formativa afeta o interesse, a atitude e as emoções dos alunos que participam do grupo tutorial. A pesquisa caracteriza-se como qualitativa, tendo como informantes alunos e uma tutora do curso de Medicina de uma faculdade do interior da Bahia. Para coleta de dados foi utilizada a Observação Participante com utilização de questionários estruturados e observação da dinâmica tutorial no ano de 2021. A análise dos dados se deu através do Critério de Idoneidade Didática Emocional adaptados para o curso de Medicina, que tem a finalidade de medir como discentes se interessam e são afetados no processo ensino-aprendizagem. Como resultado percebeu-se que os discentes e docente ainda valorizam mais a avaliação somativa em detrimento à formativa, criando comportamentos aversivos ao processo avaliativo, tais como ansiedade e medo. Percebeu-se a noção da importância da avaliação ao final da tutoria como forma de estimular o desenvolvimento de habilidades e atitudes, embora ela não siga uma padronização de autoavaliação, avaliação dos pares, avaliação do tutor e avaliação pelo tutor.

Resumo: As histórias de vida de pessoas trans-masculinas, embora diversificadas, demonstram que a construção da masculinidade não pode ser entendida fora dos efeitos materiais produzidos no e pelo corpo, dentro de uma ordem de género erigida sobre a masculinidade hegemónica. Confrontados com a centralidade dada ao corpo por indivíduos trans-masculinos em Portugal e no Reino Unido, exploramos as ligações entre masculinidade, incorporação e a materialidade corporal do género. Seguindo Raewyn Connell (1995, p. 56), quando afirma a inevitabilidade do corpo na construção da masculinidade, desenvolvemos duas linhas de argumentação. Primeiro, sugerimos que a reconfiguração das premissas conceptuais da masculinidade, como lugar, prática e efeito, é um passo fundamental para entender as possibilidades de se fazer masculinidade. Segundo, defendemos que a masculinidade é, sobretudo, um efeito de práticas corporais reflexivas.

Abstract: The life stories of trans-masculine individuals, though diverse, demonstrate that the construction of masculinity cannot be understood outside the material effects produced in and by the body, within a gender order erected on hegemonic masculinity. Confronted with the centrality given to the body by trans-masculine individuals in Portugal and the UK, we explore the links between masculinity, embodiment and the bodily materiality of gender. Following Raewyn Connell (1995, p. 56) when she asserts the inevitability of the body in the construction of masculinity, we develop two lines of argument. First, we suggest that reconfiguring the conceptual premises of masculinity as place, practice and effect is a key step in understanding the possibilities of doing masculinity. Second, we argue that masculinity is, above all, an effect of reflexive bodily practices.

Resumen: Las historias de vida de individuos trans-masculinos, aunque diversas, demuestran que la construcción de la masculinidad no puede entenderse fuera de los efectos materiales producidos en y por el cuerpo, dentro de un orden de género erigido sobre la masculinidad hegemónica. Frente a la centralidad otorgada al cuerpo por los individuos trans-masculinos en Portugal y en el Reino Unido, exploramos los vínculos entre la masculinidad, la corporeidad y la materialidad corporal del género. Siguiendo a Raewyn Connell (1995, p. 56) cuando afirma la inevitabilidad del cuerpo en la construcción de la masculinidad, desarrollamos dos líneas de argumentación. En primer lugar, sugerimos que reconfigurar las premisas conceptuales de la masculinidad como lugar, práctica y efecto es un paso clave para entender las posibilidades de hacer masculinidad. En segundo lugar, sostenemos que la masculinidad es principalmente un efecto de las prácticas corporales reflexivas.

RESUMO Introdução: Entre 2008 e 2019, o Laboratório de Histopatologia Cutânea do Serviço de Dermatologia do Hospital de Santa Maria efectuou 60 741 exames histopatológicos, dos quais 11 873 corresponderam a patologia cutânea não tumoral (20%). Material e Métodos: Realizámos um estudo retrospetivo através da análise dos exames histopatológicos da patologia cutânea não tumoral estudados no laboratório durante o períodocompreenddo entre janeiro de 2008 e dezembro de 2019. Resultados: O grupo nosológico onde se documentou maior número de diagnósticos foi o eritemas reactivos (16%), seguido das dermatoses espongióticas (14%), infeções cutâneas (14%), dermatoses psoriasiformes (10%), dermatoses liquenóides (8%), doenças bolhosas autoimunes (7%), vasculopatias (7%), colagenoses (6%), doenças granulomatosas (5%), doenças não neoplásicas dos anexos (4%), paniculites (1%), afecções não tumorais das mucosas (1%) e das genodermatoses (1%), tendo sido observados 6% de casos referentes a várias outras entidades não tumorais não enquadráveis nos grupos considerados. Conclusão: Comparativamente às séries anteriormente publicadas relativas à actividade deste laboratório (referentes aos períodos compreendidos entre 1975-1980 e 1998-2007), constatou-se que o número total de exames de doenças cutâneas não tumorais tem sido progressivamente maior. Este crescimento global verificou-se em todas as categorias patológicas, com excepção das genodermatoses, relativamente às quais observou-se um decréscimo do número de exames realizado. Os dados observados consolidam o papel do Laboratório de Histopatologia Cutânea no âmbito da actividade assistencial realizada no Serviço de Dermatologia do Hospital de Santa Maria, não só pelo progressivo aumento de casuística nele processada, como também pelo maior nível de diferenciação alcançado.

ABSTRACT Introduction: Between 2008 and 2019, in the Skin Pathology Laboratory of the Dermatology Department of Hospital de Santa Maria (Lisbon), 60 741 histological exams were performed, 11 873 of them concerned non tumour diseases (20%). Material and Methods: A retrospective study was conducted through the analysis of the histological exams of the non-tumoral skin pathology of the laboratory between January 2008 and December 2019. Results: Toxic erythemas were the most frequent group diagnosed (16%), followed by spongiotic dermatosis (14%), cutaneous infections (14%), psoriasiform dermatosis (10%) lichenoid dermatosis (8%), autoimmune bullous skin diseases (7%), vasculopathies (7%), collagenosis (6%), granulomatous diseases (5%), non tumour skin adnexal diseases (4%), panniculitis (1%), non tumour mucosal diseases (1%) and genodermatoses (1%). A number of cases (6%) corresponded to many diverse diagnoses that could not be classified into the main groups considered. Conclusion: Comparing with two previous series concerning the activity of this laboratory (referring to the period between 1975-1980 and 1998-2007), it was noticed that the total number of exams that were done in the considered period had consistently grown. This global growth was seen in all pathological categories, with the exception of genodermatoses. The observed results reaffirm the relevant role of the Skin Pathology Laboratory in the context of the Dermatology Department of Hospital de Santa Maria (Lisbon), not only based on the volume of exams there processed but also due to the higher level of expertise it achieved.

RESUMO: Introdução: O Laboratório de Histopatologia Cutânea do Serviço de Dermatologia do Hospital de Santa Maria de Lisboa funciona ininterruptamente desde sua criação em 1975. Material e Métodos: Realizámos um estudo retrospetivo através da análise dos exames histológicos da patologia cutânea tumoral diagnosticados no laboratório durante o período de janeiro de 2008 a dezembro de 2019. Resultados: No período 2008-2019 o Laboratório de Histopatologia Cutânea efetuou 60 741 exames histológicos, dos quais 80% foram lesões tumorais. Destes, os tumores malignos representaram cerca de um terço da amostra, essencialmente constituída por basaliomas (53%), carcinomas espinocelulares (23%) e melanomas (8%). Outros tumores malignos documentados foram os linfomas (3%), o sarcoma de Kaposi (1%) e as infiltrações da pele por doenças malignas extracutâneas (2%). Do grupo das neoplasias benignas (68% do número total dos tumores) destacam-se os nevos melanocíticos (50%), as verrugas seborreicas (16%) e os quistos (6%). Conclusão: Em termos comparativos com anteriores casuísticas, tem-se assistido a um aumento constante e regular da actividade geral do Laboratório de Histopatologia Cutânea do Serviço de Dermatologia do Hospital de Santa Maria nos últimos 12 anos. Isto tem sido refletido num aumento no número de diagnósticos de tumores cutâneos malignos. Destacamos a importância do exame histológico como componente central no diagnóstico e subsequente tratamento das condições neoplásicas da pele.

ABSTRACT: Introduction: The Skin Pathology Laboratory of the Department of Dermatology of Hospital de Santa Maria, Lisbon, has been operating continuously since its creation. Material and Methods: A retrospective study was conducted through the analysis of the histological exams of the tumoral skin pathology of the Laboratory between January 2008 and December 2019. Results: In the period 2008-2019 the Skin Pathology Laboratory performed 60 741 histological exams, of which 80% were tumoral lesions. Of these, malignant tumors represented about one third of the sample, essentially constituted by basal cell carcinomas (53%), squamous cell carcinomas (23%) and melanomas (8%). Other malignant tumors were lymphomas (3%), Kaposi's sarcoma (1%) and skin disorders due to extracutaneous malignancies (2%). The group of benign neoplasms (68% of the total number of tumors) included melanocytic nevi (50%), seborrheic keratosis (16%) and cysts (6%). Conclusion: Comparing with former casuistic studies, a steady and regular increase in the general activity of the Laboratory of Cutaneous Histopathology at the Dermatology Service of Hospital de Santa Maria has been observed in the past 12 years. This has been reflected in an increase in the number of diagnoses of malignant skin tumors. We highlight the value of histological examination as a central component in the diagnosis and subsequent treatment of neoplastic skin conditions.

Abstract INTRODUCTION: Since 2016, Brazil has been in the midst of its largest sylvatic yellow fever epidemic ever, found predominantly outside the Amazon region. Cases originating from Brazil have been reported in France, the Netherlands, Romania, Switzerland, Argentina, and Chile. The epidemic began in the Central-West region of Brazil in 2014, spreading into the Southern region, with significant non-human primate transmission continuing towards Paraguay and Argentina. METHODS: This report is an integrative review of Pan American Health Organization cooperation during a sylvatic yellow fever epidemic. RESULTS: The Pan American Health Organization has played a central role in handling the yellow fever emergency, collaborating with the Ministry of Health and various research groups in supporting interventions of different response areas. The Pan American Health Organization's technical cooperation included: training and workshops to exchange experiences, carrying out technical cooperation in patient management and epidemiological, entomological, laboratory, and epizootic surveillance, organizing the assistance network, and acquiring strategic inputs. The Pan American Health Organization’s technical cooperation supported the Ministry of Health’s decision to adopt a single-dose vaccine and use fractional doses to support the vaccination needs of more than 39,000,000 people. The coronavirus disease 2019 pandemic contributed to the failure of reaching the yellow fever vaccination goals and made it difficult to integrate the yellow fever vaccine into recommended areas. CONCLUSIONS: Given the ongoing coronavirus disease 2019 pandemic, it is necessary to strengthen measures for the surveillance, prevention, and control of yellow fever with multilateral cooperation between countries.

Resumo: Introdução: No contexto das graduações médicas, o desenvolvimento de metodologias que estimulam a busca do aprendizado, a autonomia e a criatividade dos discentes é essencial para a formação médica no Brasil. O estudo visa descrever a construção de infográficos como proposta pedagógica para o aprendizado dos processos orgânicos do envelhecimento humano por estudantes de Medicina. Método: Os discentes do quarto período do curso de Medicina de uma instituição de ensino superior construíram infográficos como requisito de conteúdo prático do módulo “Processos de Envelhecimento”. Adotou-se o infográfico do tipo estático, seguindo critérios como: definição do público-alvo; definição do objetivo; escolha do tema; seleção das informações mais relevantes (foco); linguagem direta e acessível; informações organizadas; escolhas das paletas de cores e estilo; e esboço do infográfico. Todo processo de elaboração foi supervisionado pelo docente responsável, sendo previamente estabelecidos os critérios de avaliação. Resultados: A turma foi dividida em sete grupos, resultando na produção de um infográfico com uma temática específica por grupo. Os temas do envelhecimento humano foram: doenças articulares degenerativas, enfraquecimento ósseo, pneumonia no idoso, infarto agudo do miocárdio, demência vascular, aterosclerose e herpes-zóster. É válido salientar que, além da criação, cada grupo apresentou aos demais colegas o produto final, explicitando cada item inserido no infográfico estático. Conclusões: Foi possível observar que os alunos cumpriram de forma satisfatória os requisitos avaliativos propostos, demonstrando envolvimento na construção dos infográficos e, sobretudo, no aprendizado simples, criativo e objetivo, utilizando uma poderosa ferramenta visual. Acrescenta-se também que o material impresso servirá de apoio no laboratório de histologia e em atividades extramuros.

Abstract: Introduction: In the context of medical school, the development of methodologies that stimulate the students’ search for learning, autonomy and creativity are essential for medical education in Brazil. The study aims to describe the construction of infographics as a pedagogical proposal for the learning of organic human aging processes by medical students. Method: Medical students attending the 4th period at a Higher Education Institution built infographics, as a requirement for the practical content of the Aging Process module. The static-type infographic was adopted, following criteria such as the definition of the target audience; definition of the objective; choice of topic; selection of the most relevant information (focus); direct and accessible language; organized information; choices of color palettes and style and; infographic sketch. The entire creation process was supervised by the teacher in charge of the project, and evaluation criteria were previously established. Results: The class was divided into seven groups, resulting in the production of an infographic with a specific topic per group. The human aging topics were: Degenerative Joint Diseases, Bone Weakness, Pneumonia in the Elderly, Acute Myocardial Infarction, Vascular Dementia, Atherosclerosis and Herpes Zoster. It is worth noting that in addition to the creation, each group presented the final product to the other colleagues, explaining each item included in the static infographic. Conclusions: We observed that the students satisfactorily met the proposed evaluation requirements, demonstrating their involvement in the construction of infographics and, above all, in simple, creative and objective learning, using a powerful visual tool. We also add that the printed material will be used as aid in the histology laboratory and in extramural activities.

BACKGROUND Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi). METHODS Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed by RT-PCR, immunohistochemistry and neutralisation test, respectively. FINDINGS Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi); in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman’s capsule and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi), in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi). MAIN FINDINGS Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore, constitute an excellent experimental model for studies on the pathophysiology of the disease.

Abstract: We review the potential of Amazon forest as a source for circulation and maintenance of native arboviruses as well its capacity to host exotic arboviruses introduced in Brazil during their process of adapting to the Amazon environment. After a brief introduction about arboviruses isolated in Amazon region and description of the main arboviruses pathogenic to humans, we highlight the history of the last two exotic viruses introduced in Brazil - Chikungunya virus (CHIKV) and Zika virus (ZIKV) - and their consequences to the public health. Finally, we discuss and hypothesize what will happen with them after the outbreak. We look to the past to predict the future.

Abstract INTRODUCTION: Dengue is an important mosquito-borne disease in tropical and subtropical regions. Adhesion molecules have not been systematically characterized in the renal tissue of patients with severe dengue (SD). The objective of this study was to detect viral antigens in samples from patients that evolved with SD, correlating with the expression of ICAM-1, VCAM-1, VE-cadherin, and E-selectin to contribute to a better understanding of the pathophysiology of SD. METHODS: Kidney specimens from patients with SD were selected according to clinical and laboratorial data and submitted to histological and immunohistochemistry analysis. A semiquantitative evaluation was performed considering positive immunostaining in 20 glomeruli. RESULTS: Viral antigens were mainly detected in distal tubules. The intense immunostaining of VCAM-1 and ICAM-1 was observed. The expression of E-selectin was discrete, and VE-cadherin expression varied from mild to moderate. VCAM-1 was slightly intense in the glomerular capsule; the expression of ICAM-1 was diffuse. E-selectin was diffuse, and VE-cadherin varied from mild to moderate. The most frequent histological findings were glomerular congestion, mild glomerulitis, acute renal injury, and glomerular atrophy. CONCLUSIONS: The results appear to demonstrate an imbalance between vascular endothelial permeability regulating events in renal lesions in SD. The increase in the expression of ICAM-1 and VCAM-1 is an in-situ indicator of higher permeability with a consequent influx of cells favoring the inflammation of the endothelium. These molecules are important in the pathophysiology of the disease and provide the possibility of developing new markers for the evaluation, clinical follow-up, and therapeutic response of patients with SD.

BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000’s decade.

Abstract We report a case of encephalitis associated with Zika virus infection and reactivation of varicella-zoster virus in the central nervous system of a Brazilian child. This case raises the possibility that reactivation of the latent varicella-zoster virus may be a mechanism of neurological impairment induced by acquired Zika virus infection.

Abstract A sulfated polysaccharide fraction from the red alga Gracilaria intermedia (PLS) was obtained by papain digestion (60 °C, 30 min). The extract was subjected to colorimetry and turbidimetry analysis, Fourier transform infrared (FTIR) spectroscopy, 1H, 13 C and 2D 1H COSY nuclear magnetic resonance (NMR) and gas chromatography/mass spectrometry analysis. Antioxidant activity tests were performed (chelation of ferrous ion, total antioxidant capacity, and scavenging of DPPH radicals); significant activity of the extract indicated that these polysaccharides may be used as non-synthetic antioxidants. The rheological behavior of aqueous polysaccharide solutions was studied at 25 ± 1 °C using steady-shear and dynamic oscillatory measurements. All the solutions analyzed showed pseudoplastic behavior and potential to act as a thickening agent, as proved through a preliminary comparison with a commercial product used for this application.

The yellow fever (YF) vaccine has been used since the 1930s to prevent YF, which is a severe infectious disease caused by the yellow fever virus (YFV), and mainly transmitted by Culicidae mosquitoes from the genera Aedes and Haemagogus . Until 2013, the World Health Organization (WHO) recommended the administration of a vaccine dose every ten years. A new recommendation of a single vaccine dose to confer life-long protection against YFV infection has since been established. Recent evidence published elsewhere suggests that at least a second dose is needed to fully protect against YF disease. Here, we discuss the feasibility of administering multiple doses, the necessity for a new and modern vaccine, and recommend that the WHO conveys a meeting to discuss YFV vaccination strategies for people living in or travelling to endemic areas.

This study showed that laboratory markers of recent infection by dengue, Zika or chikungunya arboviruses were detected in the biological samples of approximately one-third of patients with encephalitis, myelitis, encephalomyelitis or Guillain-Barré syndrome, in a surveillance programme in Piauí state, Brazil, between 2015-2016. Fever and myalgia had been associated with these cases. Since in non-tropical countries most infections or parainfectious diseases associated with the nervous system are attributed to herpesviruses, enteroviruses, and Campylobacter jejuni, the present findings indicate that in tropical countries, arboviruses may now play a more important role and reinforce the need for their surveillance and systematic investigation in the tropics.

BACKGROUND In this study, we evaluated the role of free-living domestic pigeons (Columba livia) as a reservoir of arboviruses in the city of Belém, state of Pará, Brazil. We investigated the presence of antibodies against the most prevalent arboviruses. OBJECTIVES This study was aimed at evaluating some clinical and physical parameters of domestic pigeons, including the presence of antibodies to Amazon-endemic arboviruses. METHODS Eighty-five healthy pigeons were captured in Mangal das Garças Park, in Belém, and were bled. Upon capture, the birds were subjected to a clinical examination in search of alterations that could indicate the presence of arboviruses. Blood samples were converted to serum and tested using the haemagglutination inhibition (HI) technique with a panel of 19 antigens of arboviruses circulating in the Amazon. The confirmation assay for the positive reactions to the viral species tested by HI was a neutralisation test in new-born Swiss albino mice (Mus musculus) [mouse neutralisation test (MNT)]. FINDINGS A total of 10 (11.8%) serum samples tested positive for antiflavivirus antibodies by HI. All the samples positive for the HI test were subjected to MNT for detection of viruses and yielded negative results (logarithmic neutralisation index < 1.7). MAIN CONCLUSION The results represent the first serological detection of antiarbovirus antibodies in domestic pigeons as potential hosts of arboviruses in Brazil. The detection of haemagglutination-inhibiting antibodies against genus Flavivirus indicated that there was recent contact between the analysed domestic pigeons and these arboviruses. Further studies are needed to evaluate the role of free-living pigeons in the maintenance cycle and spread of arboviruses in the Amazon.